Occurrence of IgA and IgG autoantibodies to calreticulin in coeliac disease and various autoimmune diseases

Calreticulin (CRT), a high-affintiy calcium binding protein and chaperone, was recently identified as one of the targets of autoantibodies in coeliac disease. We evaluated the level of IgA and IgG antibodies to CRT in sera fr om patients with coeliac disease and various autoimmune diseases. The level of antibodies to gliadin (shown previously to cross-react with CTR), isola ted enterocytes and tissue transglutaminase were determined for comparison. The mean level of IgA antibodies to CRT was significantly higher (P < 0.00 1) in sera from coeliac patients with active disease (139.9 +/- 11.2 AU/+/- SE) than in healthy controls (20.9 +/- 1.7 AU). In sera of patients with sy stemic lupus erythematosus (SLE), insulin dependent diabetes mellitus (IDDM ), multiple sclerosis (MS) and autoimmune thyroiditis (AT) or inflammatory bowel disease (IBD) the mean level (25.8 +/- 3.7 to 38.1 +/- 5.6 AU) did no t exceed the cut-off value. A low level of these antibodies, however, was d etected in some sera of patients with MS and IBD. The level of IgG anti-CRT antibodies was increased in coeliac patients (mean 125.4 +/- 8.0 AU, P < 0 .001) when compared to that in healthy controls (33.9 +/- 2.3 AU). The IgG anti-CRT antibodies were also detected in about 30% of SLE patients sera (5 4.1 +/- 3.6 AU, P < 0.001), but the mean level reached only half that detec ted in coeliac patients, (C) 2000 Academic Press.