Study of cell-lipid film interaction by measuring heat shock proteins mRNAexpression

The adhesion and function of HeLa S3 cells on lipid films were investigated . The function of the cells adhering to lipid films was estimated by HSP 70 B and HSP 47 mRNA expression using a reverse transcription-polymerase chain reaction (RT-PCR) analysis. It is apparent that HeLa S3 cells adhere to li pid films as well as tissue culture poly(styrene)(TCPS). HSPs mRNA expressi on on the lipid film depended on each lipid film. HeLa S3 cells adhered to the lipid films of DPPC, DPPE and DPPS expressed the same level HSPs mRNA a s TCPS. High HSPs mRNA expressions were observed in the HeLa S3 cells adher ing to films of synthetic lipids. The serum proteins affected the HSPs expr ession in the case of the synthetic lipids. These results imply that cell-m aterial interactions are not simple and that mRNA expression is a powerful tool in the investigation of cell. function, especially the reaction by wea k stimulus such as cell-material contact.