The Saccharomyces cerevisiae ATP synthase

The ATP synthase of the yeast Saccharomyces cerevisiae is composed of 20 di fferent subunits whose primary structure is known. The organization of prot eins that constitute the membranous domain is now under investigation. Cyst eine insertions combined with the use of nonpermeant maleimide reagents and cross-linking reagents showing different lengths and specificity contribut e to the knowledge of the location of the N- and C-termini of the subunits involved in the stator of the enzyme and their organization. This review su mmarizes data on yeast ATP synthase obtained in our laboratory since 1980.