Identification of positive and negative determinants of malonyl-CoA sensitivity and carnitine affinity within the amino termini of rat liver- and muscle-type carnitine palmitoyltransferase I

The extreme amino terminus and, in particular, residue Glu-3 in rat liver ( L) carnitine palmitoyltransferase I (CPT I) have previously been shown to b e essential for the sensitivity of the enzyme to inhibition by malonyl-CoA. Using the Pichia pastoris expression system, we now observe that, although mutants E3A (GIu-3 --> Ala) or Delta (3-18) of L-CPT I have markedly lower ed sensitivity to malonyl-CoA compared with the wild-type protein, the muta nt Delta (1-82) generated an enzyme that had regained much of the sensitivi ty of wild-type CPT I. This suggests that a region antagonistic to malonyl- CoA sensitivity is present within residues 19-82 of the enzyme. This was co nfirmed in the construct Delta (19-30), which was found to be 50-fold more sensitive than wild-type L-CPT I, Indeed, this mutant was >4-fold more sens itive than even the native muscle (M)-CPT I isoform expressed and assayed u nder identical conditions. This behavior was dependent on the presence of G lu-3, with the mutant E3A-Delta (19-30) having kinetic characteristics simi lar to those of the E3A mutant. The increase in the sensitivity of the L-CP T I-Delta (19-30) mutant was not due to a change in the mechanism of inhibi tion with respect to palmitoyl-CoA, nor to any marked change of the K-0.5 f or this substrate. Conversely, for M-CPT I, a decrease in malonyl-CoA sensi tivity was invariably observed with increasing deletions from Delta (3-18) to Delta (1-80). However, deletion of residues 3-18 hom M-CPT I affected th e II;, for carnitine of this isoform, but not of L-CPT I, These observation s (i) provide the first evidence for negative determinants of malonyl-CoA s ensitivity within the amino-terminal segment of L-CPT I and (ii) suggest a mechanism for the inverse relationship between affinity for malonyl-CoA and for carnitine of the two isoforms of the enzyme.