In vivo and in vitro degradation of glucagon-like peptide-2 in humans

Glucagon-like peptide-2 (GLP-2), an intestinal product of glucagon gene exp ression which induces intestinal growth in mice, has been proposed as a tre atment for intestinal insufficiency. GLP-2 is metabolized extensively by di peptidyl peptidase TV (DPP-IV) in rats, but less is known about its fate in humans. Therefore, GLP-2 metabolism was investigated in healthy volunteers alter 1) a 500-Cal mixed meal (n = 6)1 2) iv infusion of synthetic human G LP-2 (0.8 pmol/kg min; n = 8), 3) a sc bolus injection (400 mug; n = 9), an d 4) in vitro incubation in plasma and blood (1000 pmol/L; n = 4). GLP-2 co ncentrations were determined by N-terminal RIA measuring only intact GLP-2, side-viewing RIA. measuring intact and degraded forms [e.g. GLP-2-(3-33) a rising from DPP-TV degradation], and high performance liquid chromatography (HPLC). Meal ingestion elevated plasma GLP-2 (intact, 16 +/- 3 to 73 +/- 1 0 pmol/L at 90 min), and HPLC revealed two immunoreactive components: intac t GLP-2 (57 +/- 2%) and GLP-2-(3-33). GLP-2 infusion increased plasma level s [intact, 9 +/- 4 to 131 +/- 11 pmol/L; total, 23 +/- 7 to 350 +/- 18 pmol /L; the differences represent GLP-2-(3-33)]. The elimination t(1/2) values were 7.2 +/- 2 min (intact GLP-2) and 27.4 +/- 5.4 min [GLP-2-(3-33)], and MCRs were 6.8 +/- 0.6 and 1.9 +/- 0.3 mL/kg.min, respectively. Subcutaneous injection increased intact GLP-2 to maximally 1493 +/- 250 pmol/L at 45 mi n, whereas total GLP-2 increased to 2793 +/- 477 pmol/L at 90 min. At 60 mi n, plasma contained 69 +/- 1% intact GLP-2. In vitro the t(1/2) values were 8.0 +/- 1.5 h (plasma) and 3.3 +/- 0.3 h (blood). GLP-2-(3-33) was the onl y degradation product identified by HPLC, and a DPP-IV inhibitor abolished the degradation of GLP-2 in vitro. We conclude that GLP-2 is extensively de graded to GLP-2-(3-33) in humans, presumably by DPP-IV. Nevertheless, 69% r emains intact 1 h after GLP-2 injection, supporting the possibility of sc u se in patients with intestinal insufficiency.