Characterization of the gene encoding a histidine and aspartic acid-rich protein from Pneumocystis carinii

Authors
Lee, CH Lasbury, ME Paulsrud, JR Bauer, NL Brady, SL Weinberg, GA Durant, PJ Bartlett, MS Smith, JW
Citation
Ch. Lee et al., Characterization of the gene encoding a histidine and aspartic acid-rich protein from Pneumocystis carinii, J EUKAR MIC, 47(6), 2000, pp. 581-584
Citations number
21
Categorie Soggetti
Biology,Microbiology
Journal title
JOURNAL OF EUKARYOTIC MICROBIOLOGY
ISSN journal
10665234 → ACNP
Volume
47
Issue
6
Year of publication
2000
Pages
581 - 584
Database
ISI
SICI code
1066-5234(200011/12)47:6<581:COTGEA>2.0.ZU;2-Y
Abstract
A cDNA clone derived from Pneumocystis carinii contained an unusual sequenc e (GTGATG)(2)(ATGGTG)(4)(ATG)(4) and many GAT repeats. It was found to enco de a histidine and aspartic acid-rich protein (HARP). The complete cDNA con tained an 888-bp open reading frame encoding a putative protein of 32.6 kDa . The deduced HARP protein contained 39 aspartic acid and 22 histidine resi dues. The genomic copy of the HARP gene (1203 bp in length) was found to co ntain 3 small introns of 46, 44, and 38 bp, respectively. HARP was predicte d by computer programs to be a plasma membrane protein with nickel-binding activity.