The pacemaker activity generating the intrinsic myogenic contraction of the dorsal vessel of Tenebrio molitor (coleoptera)

Combined intracellular and extracellular recordings from various parts of t he isolated dorsal vessel of Tenebrio molitor revealed some of the followin g electrophysiological properties of the heart and the aorta. (i) The wave of depolarization causing forward pulsation of the dorsal vessel was always transmitted from posterior to anterior, with a conduction velocity of 0.01 4ms(-1) in the heart and 0.001ms(-1) in the aorta when the heart rate was 6 0 beats min(-1). (ii) There: was no pacemaker activity in the aorta. (iii) The duration of the compound action potential in the aortic muscle depended on the duration of the pacemaker action potential generated in the heart. (iv) Isolated parts of the heart continued to contract rhythmically for hou rs, indicating powerful pacemaker activity in individual cardiac segments. (v) There was a direct relationship between action potential duration and t he length of the preceding diastolic interval. (vi) The rhythmic wave of de polarization was dependent on the influx of Ca2+. (vii) The recovery of the electrical properties of myocardial cells that had been disrupted by secti oning was rapid. (viii) In hearts sectioned into two halves, the rhythmic p acemaker action potentials recorded simultaneously from the two isolated ha lves eventually drifted out of phase, but they had the same intrinsic frequ ency. In the light of these data, we discuss two alternative models for the generation of spontaneous rhythmic pumping movements of the heart and aort a.