Simultaneous analysis of streptomycin, dihydrostreptomycin and their related substances by capillary zone electrophoresis

Citation
Ym. Li et al., Simultaneous analysis of streptomycin, dihydrostreptomycin and their related substances by capillary zone electrophoresis, J LIQ CHR R, 23(19), 2000, pp. 2979-2990
Citations number
14
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
10826076 → ACNP
Volume
23
Issue
19
Year of publication
2000
Pages
2979 - 2990
Database
ISI
SICI code
1082-6076(2000)23:19<2979:SAOSDA>2.0.ZU;2-6
Abstract
A capillary zone electrophoresis method was developed and validated for sim ultaneous separation of streptomycin (STM) and dihydrostreptomycin (DHS) wi th direct UV detection at 205 nm. Good selectivity was obtained within a sh ort analysis time. Electrophoresis conditions were as follows: 50 mM sodium tetraborate includ ing 5% (v/v) methanol at pH 10.25; fused-silica capillary, 44 cm (effective length 36 cm) x 50 mum I.D.; voltage, 15 kV; temperature, 25 degreesC. The method allows STM and DHS to be completely separated from their main relat ed substances and each other, The robustness of the method was examined by means of a full-fraction facto rial design to test the influences of buffer pH, concentration of buffer an d of methanol. The parameters for validation such as linearity, precision, limit of detection, and limit of quantitation are also reported.