Quantitation of leuprolide acetate by high performance liquid chromatography

Authors
Singh, J Rastogi, SK Singh, SN Bhatia, JS
Citation
J. Singh et al., Quantitation of leuprolide acetate by high performance liquid chromatography, J LIQ CHR R, 23(19), 2000, pp. 3023-3031
Citations number
6
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
10826076 → ACNP
Volume
23
Issue
19
Year of publication
2000
Pages
3023 - 3031
Database
ISI
SICI code
1082-6076(2000)23:19<3023:QOLABH>2.0.ZU;2-V
Abstract
An isocratic technique was developed for the analysis of leuprolide acetate by high performance liquid chromatography (HPLC) using 220 nm UV detection , C18MICROSORB-MV(TM) column (4.6 mm x 15 cm), mobile phase (0.03 M, 77% di basic ammonium phosphate buffer: 23% acetonitrile), and 2.0 mL/min flow rat e. The coefficient of variation (C.V.) for precision and proportionality assay s was lower than 2% for all concentrations studied. The detection limit and tailing factor for leuprolide acetate were 100 ng/mL (signal-to-noise rati o of 3:1) and 1.16-1.26, respectively.