Simultaneous detection of gfp-marked Moraxella sp G21r and lux-marked Ralstonia eutrophas H850Lr using most-probable-number method

Authors
So, JS Chang, WS Leung, KT Lee, H Trevors, JT
Citation
Js. So et al., Simultaneous detection of gfp-marked Moraxella sp G21r and lux-marked Ralstonia eutrophas H850Lr using most-probable-number method, J MICROB M, 43(2), 2000, pp. 67-72
Citations number
13
Categorie Soggetti
Biology,Microbiology
Journal title
JOURNAL OF MICROBIOLOGICAL METHODS
ISSN journal
01677012 → ACNP
Volume
43
Issue
2
Year of publication
2000
Pages
67 - 72
Database
ISI
SICI code
0167-7012(200012)43:2<67:SDOGMS>2.0.ZU;2-J
Abstract
The green fluorescent protein encoded by gfp gene and the luminescent prote in encoded by luxAB genes were used as markers to detect p-nitrophenol (PNP )-degrading Moraxella sp. G21r and polychlorinated biphenyl (PCB)-degrading Ralstonia eutrophas W850Lr cells, respectively, in mixed liquid cultures a nd in soil samples using a most-probable-number (MPN) assay. Population est imates for both sfp-marked G21r and fur-marked H850Lr by using MPN assays w ere similar to viable colony counts. The MPN assay with microtiter plates p ermitted the simultaneous detection of fluorescent and luminescent bacteria in soil samples faster than conventional plate counting. (C) 2000 Elsevier Science B.V. All rights reserved.