Genetic and pharmacological demonstration of differential recruitment of cAMP - Dependent protein kinases by synaptic activity

cAMP-dependent protein kinase (PKA) is believed to play a critical role in the expression of long-lasting forms of hippocampal long-term potentiation (LTP). Can distinct patterns of synaptic activity induce forms of LTP that require different isoforms of PKA? To address this question, we used transg enic mice that have genetically reduced hippocampal PKA activity, and a spe cific pharmacological inhibitor of PKA, Rp-cAMPS. Transgenic mice [R(AB) mi ce] that express an inhibitory form of a particular type of regulatory subu nit of PKA (type-I alpha) showed significantly reduced LTP in area CA1 of h ippocampal slices as compared with slices from wild-type mice. This impairm ent of LTP expression was evident when LTP was induced by applying repeated , temporally spaced stimulation (4 1-s bursts of 100-Hz applied once every 5 min). In contrast, LTP induced by applying just 60 pulses in a theta-burs t pattern was normal in slices from R( AB) mice as compared with slices fro m wild-type mice. We found that Rp-cAMPS blocked the expression of LTP indu ced by both spaced tetra-burst and compressed theta-burst stimulation in hi ppocampal slices of wild-type and R( AB) mice, respectively. Since Rp-cAMPS is a PKA inhibitor that is not selective for any particular isoform of PKA and these R( AB) mice show reduced hippocampal PKA activity resulting from genetic manipulation of a single isoform of PKA regulatory subunit, our da ta support the idea that distinct patterns of synaptic activity can produce different forms of LTP that significantly engage different isoforms of PKA . In particular, theta-burst LTP significantly recruits isoforms of PKA con taining regulatory subunits other than the mutant RI alpha subunit, whereas tetra-burst LTP requires PKA isoforms containing the mutant RI a subunit. Thus, altering both the total amount of imposed synaptic activity and the t emporal spacing between bursts of imposed activity may subtly modulate the PKA dependence of hippocampal LTP by engaging distinct isoforms of PKA. In a broader context, our findings suggest that synaptic plasticity in the mam malian brain might be importantly regulated by activity-dependent recruitme nt of different isoforms of key signal transduction molecules.