Resonance Raman spectroscopy of Met121Glu azurin

Authors
Webb, MA Kiser, CN Richards, JH Di Bilio, AJ Gray, HB Winkler, JR Loppnow, GR
Citation
Ma. Webb et al., Resonance Raman spectroscopy of Met121Glu azurin, J PHYS CH B, 104(46), 2000, pp. 10915-10920
Citations number
33
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
JOURNAL OF PHYSICAL CHEMISTRY B
ISSN journal
15206106 → ACNP
Volume
104
Issue
46
Year of publication
2000
Pages
10915 - 10920
Database
ISI
SICI code
1520-6106(20001123)104:46<10915:RRSOMA>2.0.ZU;2-T
Abstract
The resonance Raman spectra for Pseudomonas aeruginosa Met121Glu azurin hav e been measured at wavelengths throughout the 600-nm absorption band at low and high pH. The spectra of the mutant at pH 3.5 and 7.0 are identical. An alysis of the 600-nm absorption band and resulting resonance Raman excitati on profiles under both pH conditions indicates that the excited-state disto rtions in this mutant are smaller than those in the native protein.