Deletion mapping of 14q32 in human neuroblastoma defines an 1,100-kb region of common allelic loss

Background We analyzed lass of heterozygosity (LOH) in 54 primary neuroblas tomas (NBs) using 12 microsatellite markers on 14q32, and found that 31% (1 7/54) NBs showed LOH. Procedure. The smallest region of overlap (SRO) was i dentified between D14S62 and D14S987. Results. There was no statistical cor relation between COH and any clinicopathologic features, including age, sta ge, amplification of MYCN, and ploidy. A sequence-ready bacterial artificia l chromosome (BAC) contig was constructed, and the minimum tiling path of s ix BACs covered the SRO; the physical length of this region was no larger t han 1,000 kb. Conclusions. Our findings support the existence of a putative tumor-related gene on 14q32 for the tumorigenesis of NB. (C) 2000 Wiley-Li ss, Inc.