Differential expression in an antisense MYCN neuroblastoma model

Background. A previously published antisense MYCN-expressing model system w as utilized to identify genes whose expression is altered by the down-regul ation of MYCN by AS MYCN. Results. Differential display comparing nontransf ected human NE NBL-S cells to three AS MYCN-expressing cell lines (NBAS-4, -5, and -6) yielded nine differentially expressed cDNAs designated NDDE:1-9 , for MYCN-dependent differential expression genes. A GenBank search reveal ed matches for seven of the nine cDNAs. Differential expression was confirm ed for five of the cDNAs by Northern blot analysis. Results. NDDE:8 is up-r egulated in the AS MYCN-expressing clones and shares homology with the EB1 clone p53-induced gene (PRG3). NDDE:2 is up-regulated in the high-expressin g N-myc protein NBL-S cells and shares homology with a 27 kD heat shock pro tein PAN1 clone. Further analysis of all five cDNAs might further elucidate the targets of MYCN in NE. Med. Pediatr. Oncol. 35:669-672, 2000. (C) 2000 Wiley-Liss, Inc.