The effects of chronic exercise on anesthesia induced hepatotoxicity

A hypoxic rat model of halothane-induced hepatotoxicity, which is known to produce liver damage, was used to determine the effects of chronic exercise on halothane-induced hepatotoxicity and on reduced hepatic glutathione (GS H) levels. Metabolism of volatile anesthetics may generate metabolites that can cause mild and transient hepatotoxicity. Methods: Six male Sprague-Daw ley rats completed a IO-wk (5 d.wk(-1)) treadmill running protocol. Twelve age-matched animals were used as sedentary controls. After the completion o f exercise training, rats were exposed for 2 h to 1% halothane in 14% O-2. Twenty-four hours later, animals were anesthetized with sodium pentobarbita l and sacrificed. Livers were excised, stained, and evaluated for hepatotox icity using a histopathological 0 (normal) to 5 (severe damage) point categ orical scale and for the determination of GSH levels. Results: Median histo pathologic scores revealed significantly lower indications of hepatotoxicit y in exercise animals as compared with control animals (score = 0.25 vs 1.5 0; P < 0.05). Liver damages scores between 1 and 5 were observed in 75% (9 of 12) of the control animals, whereas only 1 of 6 exercise animals had a s core greater than 1 (P < 0.05). No significant difference was observed in r educed GSH levels. Conclusions: Chronic exercise improves the detoxicant ab ility of the liver for halothane anesthesia as noted by the ameliorated liv er damage and reduced incidence of halothane-induced hepatotoxicity in the exercise animals.