The use of porous silicon (FS) as an efficient surface enlarging carrier ma
trix fur immobilised enzymes is demonstrated. An optimal porous matrix in p
-type silicon (10-20 Omega cm) with respect to enzymatic substrate turn-ove
r was obtained when anodising the sample at 100 mA/cm(2) for 5 min. An incr
ease in glucose turn-over of approximate to 220 times as compared to an enz
yme activated polished surface was recorded. The hight st increase in turn-
over was found to be 350 times for an n-epilayer on n(+) substrate. The app
lication of glucose monitoring is demonstrated showing a linear range to 15
mM glucose with a satisfactory storage and operational stability. The use
of ascorbate oxidase activated porous silicon for the elimination of ascorb
ate tan electrochemical interferent) in glutamate monitoring is reported. A
n upper elimination level of I mM ascorbate was found. PS is also reported
as an efficient protein cleavage surface when activated with proteases. Cle
avage times normally ranging between 6 and 24 h were found to be 60 s in th
e microreactor. Myoglobin cleaved on a trypsin microreactor is shown with t
he corresponding mass spectra having a sequence coverage of 79%.