Disruption of bakers' yeast using a disrupter of simple and novel geometry

The performance of a novel hydraulically operated cell disrupter has been i nvestigated. The micro-organism used was bakers' yeast (Saccharomyces cerev isiae) and glucose-6-phosphate dehydrogenase (G6PDH) was used as a marker e nzyme. The yeast could be disrupted by a single pass through the apparatus and the enzyme release was a function of the operating pressure. The equipm ent is considered to be superior to conventional homogeniser pumps due to t he limited temperature rise observed between the feed and the lysate for co mparable levels of protein release. (C) 2000 Elsevier Science Ltd. All righ ts reserved.