Immune response to Plasmodium falciparum liver stage antigen-1: geographical variations within Central Africa and their relationship with protection from clinical malaria

Two populations of schoolchildren from Gabon and Cameroon were tested in 19 95 for their immunological reactivity to synthetic peptides (LSA-Rep, LSA-J and LSA-CTL) from Plasmodium falciparum liver stage antigen-1 (LSA-1). The prevalence and levels of both cellular (lymphocyte proliferation, tumour n ecrosis factor a (TNF alpha), interferon gamma (IFN gamma), and interleukin -10 (IL10)) and humoral (immunoglobulin G) responses were determined. Prote ction from clinical malaria, determined after a prospective 1 year study in both sites, was associated with elevated proliferative responses to LSA-Re p and LSA-CTL in the Gabonese children, as well as with higher antibody lev els to both schizont extract and LSA-Rep. The prevalence of peptide-stimula ted TNF-alpha secretion was higher in the Cameroonian group, bur higher lev els of antibodies to LSA-Rep and LSA-J were found in the Gabonese children. The immunological differences observed between children in the 2 study sit es are discussed in the context of both epidemiological and individual host factors.