Mycobacterium bovis bacillus calmette-guerin induces protective immunity against infection by Plasmodium yoelii at blood-stage depending on shifting immunity toward Th1 type and inducing protective IgG2a after the parasite infection

Bacillus calmette-guerin (BCG)-vaccination raised dramatically the survival rates of A/J mice from infection by Plasmodium yoelii 17XL at blood-stage. The analysis of the immune response of spleen cells indicated that BCG vac cination biased the immune response toward Th1 type. Neutralization of IFN- gamma and nitric oxide abrogated the protection. The kinetics of Ab product ion in the course of P. yoelii 17XL infection was monitored. Surprisingly, larger amounts of parasite-specific Abs were produced in BCC-vaccinated mic e than in the placebo control. The vast majority of the produced IgG agains t parasites in BCG-vaccinated mice was IgG2a, which was observed hardly in placebo controls. The peak of IgG2a production coincided with the clearance of infection. The naive mice transferred adoptively with IgG2a from self-c ured mice survived the lethal challenge from the parasite. These data indic ated that BCG vaccination protected A/J mouse from P. yoelii 17XL infection by biasing immunity toward Th1-type after parasite infection and enhancing production of IgG2a, which ultimately played a major role in protection. ( C) 2000 Elsevier Science Ltd. All rights reserved.