The organic matter of wastewater was fractionated into settleable (i.e., pa
rticulate) and non-settleable (i.e., colloidal+soluble) fractions by settli
ng followed by 0.22 mum filtration. Particulate, colloidal and soluble prop
ortions were found to be relatively constant (45, 31 and 24% of the total C
OD, respectively). Transfer of soluble fraction always occurred from the wa
stewater to the activated sludge flocs, whereas bidirectional transfer occu
rred for the colloidal fraction. The transfer of soluble and colloidal matt
er reached a steady state after 40 min-mixing and 20 min-mixing, respective
ly. Desorption of a part of the colloidal organic matter pre-sorbed on the
activated sludge flocs was evidenced. The biosorption capacity of activated
sludge was around 40-100 mg(COD)gT(SS)(-1). The biosorbable fraction of wa
stewater represented on average 45% of the non-settleable fraction. (C) 200
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