DIFFERENCES IN PHYCOERYTHRIN-ISOTHIOCYANATE OR FLUORESCEIN-ISOTHIOCYANATE CONJUGATED 8G12 ON CD34(+) CELL EVALUATION

Authors
ORTUNO F FERRER F LOZANO ML HERAS I MORALEDA JM VICENTE V
Citation
F. Ortuno et al., DIFFERENCES IN PHYCOERYTHRIN-ISOTHIOCYANATE OR FLUORESCEIN-ISOTHIOCYANATE CONJUGATED 8G12 ON CD34(+) CELL EVALUATION, Haematologica, 82(3), 1997, pp. 334-335
Citations number
10
Categorie Soggetti
Hematology
Journal title
Haematologica → ACNP
ISSN journal
03906078
Volume
82
Issue
3
Year of publication
1997
Pages
334 - 335
Database
ISI
SICI code
0390-6078(1997)82:3<334:DIPOF>2.0.ZU;2-X
Abstract
To evaluate the equivalence of 8G12 conjugated with either phycoerythr in (PE) or fluorescein isothiocyanate (FITC), duplicate fluorochrome-8 G12 labelling was carried out in 229 samples. Additionally, 53 samples were simultaneously immunostained with FITC-8G12 and PE-8G12. Signifi cantly higher values (p<0.001) were observed in PE-CD34(+) cells when compared with FITC-CD34(+) cells both in duplicate and simultaneous an alysis. Our data suggest that the choice of fluorochrome is relevant i n the measurement of CD34(+) cells with 8G12. (C) 1997, Ferrata Storti Foundation.