Analysis of 114 kb of DNA sequence from fission yeast chromosome 2 immediately centromere-distal to his5

One hundred and fourteen kilobase pairs (kb) of contiguous genomic sequence have been determined immediately distal to the his5 genetic marker located about 0.9 Mb from the centromere on the long arm of Schizosaccharomyces po mbe chromosome 2. The sequence is contained in overlapping cosmid clones c1 6H5, c12D12, c24C6 and c19G7, of which 20 kb are identical to previously re ported sequence from clone c21H7. The remaining 93 781 bp of sequence conta ins 10 known genes (cdc14, cdm1, cps1, gpa1, msh2, pck2, rip1, vps30-2, sad 1 and ubl1), 32 open reading frames (ORFs) capable of coding for proteins o f at least 100 amino acid residues in length, one 5S rRNA gene, one tRNA(Pr o) gene, one lone Tf1-type long terminal repeat (LTR) and one lone Tf2-type LTR. There is a density of one protein-coding gene per 2.2 kb and 22 of th e 42 ORFs (52%) incorporate one or more introns. Twenty-one of the novel OR Fs show sequence similarities which suggest functions of their products, in cluding a cyclin C, a MADS box transcription factor, mad2-like protein, tel omere binding protein, topoisomerase II-associated protein, ATP-dependent D EAH box RNA helicase, G10 protein, ubiquitin-activating el-like enzyme, nuc leoporin, prolyl-tRNA synthetase, peptidylprolyl isomerase, delta -1-pyrrol ine-5-carboxylate dehydrogenase, protein transport protein, coatomer epsilo n, TCP-1 chaperonin, beta -subunit of 6-phosphofructokinase, aminodeoxychor ismate lyase, a phosphate transport protein and a thioredoxin. The sequence has been submitted to the EMBL database under Accession Nos AL021839, AL02 2104, AL031786 and AL035085. Copyright (C) 2000 John Whey gr Sons, Ltd.