Disruption and functional analysis of six ORFs on chromosome X11 of Saccharomyces cerevisiae: YLR124w, YLR125w, YLR126c, YLR127c, YLR128w and YLR129w

In the contest of the EUROFAN programme, me report the deletion and functio nal analysis of six open reading frames (ORFs) on the right arm of chromoso me XII of Saccharomyces cerevisiae. Using a PCR-based gene replacement stra tegy, we have systematically deleted individual ORFs and subjected the hete rozygous diploids and haploid knockout strains to basic genetic and phenoty pic characterization. Two ORFs, YR127c and YLR129w, are essential for viabi lity, whereas no growth phenotype could be detected following deletion of Y LR124w, YLR125w, YLR126c or YLR128w. For each of the individual ORFs, a kan MX4 replacement cassette and the corresponding cognate mild-type gene mere cloned into appropriate plasmids, Copyright (C) 2000 John Wiley & Sons, Ltd .