Plantlets regeneration via somatic embryogenesis in immature zygotic embryos calli from a tree species adapted to arid lands: Acacia tortilis subsp raddiana (Savi.) Brenan

Using calli from immature zygotic embryos of Acacia tortilis subsp. raddian a, globular somatic embryos are obtained after 90 to 100 days culture in da rkness on VA media added with 2,4-D (2,4 and 8 mg/l) alone or in combinatio n with BAP (1 and 0,5 mg/l). Light and transfert on MS/2 medium containing BAP (0,5 mg/l)) and AIB (0,05 mg/l) enhance proliferation of somatic embryo s. ABA (2,6 and 5,2 mg/l) or high concentration of sucrose (50 g/l allow de velopment from globular to bipolar embryos. Development of mature somatic e mbryos into plantlets are obtained on MS/2 medium without hormon, but suppl emented with high concentration of sucrose.