Fluid inclusion is an efficient tracer to understand the subsurface thermal
fluid and hydrocarbon migration, however, it has some uncertainties in det
ermining maximum paleotemperature and its precise period. In order to estim
ate the reliability of homogenization temperatures, a mathematical model fo
r laboratory homogenization temperature measurement is set up based on mass
conservation theory. The formula indicates that bigger fluid inclusions ar
e more precise in determining forming temperatures. The case study of Yingq
iong Basin shows that the homogenization temperatures of the inclusions are
dominated by thermal fluid from 3000 similar to 4000m near fracture zone,
and the forming condition of the fluid inclusions indicates a close relatio
n with the overpressure beds, hydrocarbon generation, expulsion and migrati
on. This conclusion is agreeable with the observation data of vitrinite ref
lection ratio and some borehole temperatures.