Identification of 6-furfuryladenine (kinetin) in human urine

In contrast to the current view of kinetin (K, N-6-furfuryladenine) as an u nnatural and synthetic cytokinin, recently it has been identified in plant DNA and plant extract. Here we describe identification of K in human urine using chromatography/mass-spectrometry analysis for the first time. The amo unt of kinetin in urine taken from unhealthy patients lung carcinoma was es tablished to be 0.5 ng in 20 mi and a 100-fold reduced amount in healthy su bjects. Since this rare base is a potential source of structural constrains it has to be removed from DNA by enzymatic DNA-repair reactions. It seems that the presence of kinetin in human is linked to oxidative damage process es., (C) 2000 Academic Press.