Transcription modulation by a rat nuclear scaffold protein, P130, and a rat highly repetitive DNA component or various types of animal and plant matrix or scaffold attachment regions
Y. Hibino et al., Transcription modulation by a rat nuclear scaffold protein, P130, and a rat highly repetitive DNA component or various types of animal and plant matrix or scaffold attachment regions, BIOC BIOP R, 279(1), 2000, pp. 282-287
Citations number
20
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
The XmnI fragment, a highly repetitive DNA component, and animal and plant
matrix or scaffold attachment region (MAR/SAR) were examined for similarity
in interaction with nuclear scaffold. As the XmnI fragment bound a 130 kDa
scaffold protein (P130) in vitro, various types of MAR/SAR fragments could
bind 130 and 123 kDa scaffold proteins. The native XmnI and MAR/SAR fragme
nts clearly augmented SV40 promoter-mediated luciferase gene transcription
following transient transfection of recombinant plasmids into various types
of recipient cells. In contrast, the XmnI fragment methylated at the cytos
ine base of the unique HindIII site, and a synthetic variant DNA deficient
in base unpairing characteristic of MAR/ SAR, could neither bind P130 nor a
ugment this transcription. These two types of genomic regions appeared to h
ave similar properties of interaction with nuclear scaffold, by which the a
ctivity of appropriately positioned promoter can be modulated. (C) 2000 Aca
demic Press.