S. Zeppa et al., Evaluation of transcript level and biomass during mycelial development of the truffle, Tuber borchii, BIOTECH LET, 23(1), 2001, pp. 17-20
The growth of the truffle, Tuber borchii, mycelial strain (1BO) was analyse
d using biomolecular methods. Total RNA evaluation and ergosterol assay rev
ealed that transcriptional and metabolic activity reach a peak at around 30
-50 days of growth and decrease by 70 days. mRNA electrophoretic profiles h
ighlighted a very low degree of variation in gene expression during the gro
wth of mycelium.