Expression and long-term stability of a recombinant single-chain Fv antibody fragment in transgenic Nicotiana tabacum seeds

Authors
Ramirez, N Oramas, P Ayala, M Rodriguez, M Perez, M Gavilondo, J
Citation
N. Ramirez et al., Expression and long-term stability of a recombinant single-chain Fv antibody fragment in transgenic Nicotiana tabacum seeds, BIOTECH LET, 23(1), 2001, pp. 47-49
Citations number
11
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
1
Year of publication
2001
Pages
47 - 49
Database
ISI
SICI code
0141-5492(200101)23:1<47:EALSOA>2.0.ZU;2-W
Abstract
A functionally active anti-hepatitis B surface antigen single-chain Fv anti body fragment (scFv) was expressed in seeds of transgenic tobacco plants us ing genetic constructs for expression in the vacuole or the apoplastic flui d. Antibody levels close to 0.2% of the total soluble protein were found. A fter storage of the transgenic tobacco seeds for one year and a half a year at room temperature, the scFv maintained its antigen-binding activity in f ull.