Supercritical CO2 extraction of velutinol A from Mandevilla velutina (Apocynaceae) cultured cells and MALDI-TOF MS analysis

Authors
Maraschin, M Sugui, JA Wood, KV Bonham, C Lancas, FM Araujo, PS Yunes, RA Verpoorte, R Fontana, JD
Citation
M. Maraschin et al., Supercritical CO2 extraction of velutinol A from Mandevilla velutina (Apocynaceae) cultured cells and MALDI-TOF MS analysis, BIOTECH LET, 23(1), 2001, pp. 77-82
Citations number
12
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
1
Year of publication
2001
Pages
77 - 82
Database
ISI
SICI code
0141-5492(200101)23:1<77:SCEOVA>2.0.ZU;2-F
Abstract
MALDI-TOF MS analysis of supercritical CO2 extracted samples obtained from Mandevilla velutina cell cultures allowed the detection of the anti-bradyki nin pregnanic steroid, velutinol A, using low amount of sample (1 g lyophil ized cells), with minimum analyte isolation.