Role of alpha V beta 3 integrin in embryo implantation in the mouse

Integrin, a heterodimeric adhesive molecule composed of alpha and beta subu nits, can regulate cell adhesion and trafficking. Recent data have document ed that, at the "implantation window" stage, alphaV beta3 integrin particip ates in the maternal-fetal interaction and becomes a potential marker of ut erine receptivity. Furthermore, it can affect invasiveness of embryo. This work made a further study about its action mechanism. Results of indirect i mmunofluorescence and laser scanning confocal microscopy showed that alphaV beta3 integrin was clearly expressed in the mouse blastocyst. Injection of alphaV beta3 integrin antiserum into a uterine horn of a pregnant mouse on day 3 markedly decreased the number of embryos implanted (P < 0.001). In a co-culture model, <alpha>V beta3 integrin antisera at 1 : 100 and 1.200 di lutions significantly depressed the attachment and outgrowth reactions of b lastocysts on monolayer of uterine epithelial cells. Analysis of correlatio n manifested that the inhibitory effect of alphaV beta3 integrin antiserum was dosage/dilution-dependent. Thus, alphaV beta3 integrin is an essential factor in the uterine endometrium for embryo implantation in the mouse. Thi s integrin distinctly expressed in the mouse blastocyst at "implantation" s tage affected the process of embryo implantation by route of mediating both the attachment and the outgrowth processes of blastocyst on uterine epithe lial cells.