Comparison of two methods for the detection of urinary iodine used in epidemiological studies

Citation
C. Wuethrich et al., Comparison of two methods for the detection of urinary iodine used in epidemiological studies, CLIN CH L M, 38(10), 2000, pp. 1027-1031
Citations number
26
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
ISSN journal
14346621 → ACNP
Volume
38
Issue
10
Year of publication
2000
Pages
1027 - 1031
Database
ISI
SICI code
1434-6621(200010)38:10<1027:COTMFT>2.0.ZU;2-J
Abstract
Two methods for the determination of iodine in urine or serum based on the Sandell-Kolthoff reaction were compared. The first was an autoanalyser meth od (AAII, Technicon) where the mineralisation takes place in a continuous f low manner. This procedure was used at the Department of Clinical Chemistry of the University Hospital of Berne (Switzerland) from 1968 until 1993. Th e second method was evaluated and adapted in our own laboratory. Each sampl e and the iodate-standards are mineralised in a Pyrex glass tube and the de colorisation reaction takes place in a 96-well-microtiter plate which was r ead by a PC-controlled photometer at 405 nm. This method, with a detection limit of 0.1 mu mol/l showed good analytical recovery (90 to 110%) and a lo w imprecision (intra-assay coefficient of variation (CV) of 5% and an inter -assay CV of 7.5%). In contrast to the autoanalyser method, the microtiter plate-method is suitable both for series up to 24 samples (3-fold) and for single samples. A comparison of 87 samples in the range of 0.1 to 60 mu mol /l which were measured with both Sandell-Kolthoff based methods showed no o bvious discrepancy. These two methods showed a good agreement for the determination of urinary iodine. This guarantees that the results of earlier epidemiological studies can be compared with recent studies performed in our and many other labora tories.