Human oviductal cells reduce the incidence of apoptosis in cocultured mouse embryos

Objective: To investigate the effect of human oviductal cell coculture on t he incidence of apoptosis in mouse embryos. Design: Experimental laboratory study. Setting: University gynecology unit. Patient(s): Fallopian tubes were obtained from patients undergoing hysterec tomy. Intervention(s): Mouse embryos were cocultured with human oviductal cells. Main Outcome Measure(s): Blastocyst development, allocation of inner cell m ass (ICM) and trophectoderm (TE) in blastocyst, and apoptosis in embryos. Results: Oviductal cells significantly enhanced the blastulation (38%) and hatching rate (22%) of the cocultured zygotes. The corresponding values in medium alone culture were 21% and 9%, respectively. The cocultured embryos also had higher blastomere count at blastocyst stage (P<0.005). This was du e to increase in both the cell count of ICM (P<0.05) and TE (P<0.001). Cocu lture reduced the incidence of apoptosis in the cultured morula and blastoc yst from 38% and 48% to 16% (P<0.001) and 27% (P<0.05), respectively. The n umber of apoptotic blastomeres per morula (1.5 +/- 0.6; P<0.005) and blasto cyst (2.3 +/- 0.7; P<0.005) after coculture was also significantly bower th an that of the corresponding control (morula, 2.1 +/- 0.8; blastocyst, 3.5 +/- 1.1). Conclusion(s): Human oviductal cells improved mouse embryo development part ly by decreasing the incidence of apoptosis. (Fertil Steril(R) 2000;74:1215 -9. (C) 2000 by American Society for Reproductive Medicine).