Determination of idarubicin in human urine by capillary zone electrophoresis with amperometric detection

A simple, reliable and reproducible method, based on capillary zone electro phoresis with amperometric detection, has been developed for the determinat ion of idarubicin in human urine. A carbon disk electrode was used as worki ng electrode. The optimal conditions of separation and detection were pH 5. 6 phosphate buffer (0.20 mol/L), 22 kV for the separation voltage and 1.00 V (vs. Ag/AgCl, 3 mol/L KCl) for the detection potential. The linear range was from 4.0 x 10(-7) to 2.0 x 10(-5) mol/L with a regression coefficient o f 0.9986, and the detection limit was 8.0 x 10(-8) mol/L. The method was di rectly applied to the determination of idarubicin in spiked human urine wit hout any other sample pretreatment except filtration, and the assay results were satisfactory.