Adrenal cortical activation in murine colitis

Background & Aims: Proper adrenal glucocorticoid secretion is crucial in th e course of inflammatory diseases. However, the function and structure of t he adrenal glands have not been examined in inflammatory bower diseases. Me thods: After induction of trinitrobenzene sulfonic acid (TNBS) colitis in S JL/J mice, plasma hormone and cytokine levels were measured, adrenal struct ure was analyzed by immunohistochemistry and electron microscopy, and adren al cytokine/cytokine receptor expression were studied by RNase protection. Results: Adrenals of colitic animals were enlarged and hypervascularized. T hese animals had a marked increase in plasma corticosterone levels during t he course of colitis (270 +/- 34 vs. 16 +/- 11 ng/mL; P < 0.0001) but only a modest elevation of their concurrent adrenocorticotropin levels (57 +/- 1 3 vs. 29 +/- 9 pmol/L; NS). On electron microscopy, adrenocortical cells sh owed ultrastructural signs of marked stimulation, and intra-adrenal lymphoc ytes were frequently found in direct contact with these cells. Concurrent p lasma levels of interleukin (IL)-6, the major cytokine activating the hypot halamic-pituitary-adrenal axis, were markedly increased (495 +/- 131 vs. 20 +/- 1.5 pg/mL; P < 0.0001), and this cytokine directly stimulated corticos terone secretion by adrenocortical cells in vitro. Intra-adrenal expression of IL-6 in animals With colitis was increased 80-fold, and the IL-6 recept or subunits Il-6R alpha and gp130 were present in the adrenal cells. Treatm ent of animals with neutralizing anti-IL-6 antibody reduced the TNBS-induce d growth and activation of the adrenal cortices. Conclusions: Colitis is as sociated with a profound stimulation of adrenocortical cell function and gl ucocorticoid release. Direct immune-adrenal interactions seem to contribute to this activation of the adrenal glands during colitis.