E. May et al., Identical T-cell expansions in the colon mucosa and the synovium of a patient with enterogenic spondyloarthropathy, GASTROENTY, 119(6), 2000, pp. 1745-1755
intestinal T lymphocytes activated by antigen are suspected to play a key r
ole in enterogenic spondyloarthropathies (SpA). Therefore, we aimed to iden
tify and functionally characterize T-cell clones that ave coexpanded in the
intestinal mucosa and the synovium. Colon, peripheral blood, and synovium
of a patient with enterogenic SpA were screened for clones T-cell expansion
s by TCRB-CDR3 length analysis and sequencing. T-cell clones expanded in vi
vo were isolated from archived synovial cells by targeted T-cell cloning an
d characterized for phenotype, cytokine production, and antigen specificity
. The synovial TCRBV18(+) T-cell repertoire of the patient was dominated by
2 CD8(+) T-cell clones using related CDR3. Both clones were expanded throu
ghout the colon and were present in the peripheral blood. Upon in vitro sti
mulation with PDB/ionomycin, they showed predominantly interferon gamma and
interleukin (IL)-4 but also tumor necrosis factor cu and IL-10 production
and did not specifically lyse autologous T-cell blasts, B-cell lines, or ot
her autologous or allogeneic target or CD1d-transfected cells. These findin
gs strongly suggest that T lymphocytes activated by antigen in the intestin
al mucosa contribute to joint inflammation in enterogenic SPA by recognitio
n of antigens specific for the inflamed synovium.