Spectrophotometric and fluorimetric determination of amino acids by their reaction with o-phthalic aldehyde in the presence of sulfite and cyanide ions

Authors
Beketov, VI Voronina, RD Filatova, DG Zorov, NB
Citation
Vi. Beketov et al., Spectrophotometric and fluorimetric determination of amino acids by their reaction with o-phthalic aldehyde in the presence of sulfite and cyanide ions, J ANALYT CH, 55(12), 2000, pp. 1148-1151
Citations number
10
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF ANALYTICAL CHEMISTRY
ISSN journal
10619348 → ACNP
Volume
55
Issue
12
Year of publication
2000
Pages
1148 - 1151
Database
ISI
SICI code
1061-9348(200012)55:12<1148:SAFDOA>2.0.ZU;2-#
Abstract
Optimum conditions were selected for the determination of amino acids by th eir reaction with o-phthalic aldehyde in the presence of sodium sulfite and potassium cyanide using glycine as an example. It was shown that the analy tical characteristics of the proposed procedure are highly competitive with those of a widely employed procedure using 2-mercaptoethanol and that the stability of the analytical form (substituted isoindols) was much higher.