We have determined crystal structures of Sec4, a member of the Rab family i
n the G protein superfamily, in two states: bound to GDP, and to a non-hydr
olyzable GTP analog, guanosine-5'-(beta,gamma)-imidotriphosphate (GppNHp).
This represents the first structure of a Rab protein bound to GDP. Sec4 in
both states grossly resembles other G proteins bound to GDP and GppNHp. In
Sec4-GppNHp, structural features common to active Rab proteins are observed
. In Sec4-GDP, the switch I region is highly disordered and displaced relat
ive to the switch I region of Ras-GDP. In two of the four molecules of Sec4
-GDP in the asymmetric unit of the Sec4-GDP crystals, the switch II region
adopts a conformation similar to that seen in the structure of the small G
protein Ran bound to GDP. This allows residues threonine 76, glutamate 80,
and arginine 81 of Sec4 to make contacts with other conserved residues and
water molecules important for nucleotide binding. In the other two molecule
s in the asymmetric unit, these interactions do not take place. This struct
ural variability in both the switch I and switch II regions of GDP-bound Se
c4 provides a possible explanation for the high off-rate of GDP bound to Se
c4, and suggests a mechanism for regulation of the GTPase cycle of Rab prot
eins by GDI proteins. (C) 2000 Academic Press.