A peptide derived from activity-dependent neuroprotective protein (ADNP) ameliorates injury response in closed head injury in mice

Brain injury induces disruption of the blood-brain barrier, edema, and rele ase of autodestructive factors that produce delayed neuronal damage. NAPSVI PQ (NAP), a femtomolar-acting peptide, is shown to be neuroprotective in a mouse model of closed head injury. NAP injection after injury reduced morta lity and facilitated neurobehavioral recovery (P< 0.005). Edema was reduced by 70% in the NAP-treated mice (P<0.01). Furthermore, in vivo magnetic res onance imaging demonstrated significant brain-tissue recovery in the NAP-tr eated animals. NAP treatment decreased tumor necrosis factor-alpha levels i n the injured brain and was shown to protect pheochromocytoma (PC12 cells) against tumor necrosis factor-alpha -induced toxicity. Thus, NAP provides s ignificant amelioration from the complex array of injuries elicited by head trauma.