Quantification of Staphylococcus aureus cell surface adhesins using flow cytometry

The initiation of many infectious diseases involves specific adhesion of ba cteria to host tissue proteins and carbohydrates. Staphylococcus aureus is known to bind specifically to several proteins in the extracellular matrix (ECM). We report the quantification of the collagen and fibronectin adhesin densities on the staphylococcal surface using flow cytometry. Our results are in agreement with previous reports on the transcription of the respecti ve genes and demonstrate different patterns of temporal expression for the two adhesins in the strains studied. We demonstrate a convenient technique for quantification of bacterial adhesins that can be used in studies aimed at characterization of bacterial adhesion to ECM components and understandi ng expression of adhesins during the course of an infection. (C) 2000 Acade mic Press.