Inhibin and activin subunits are differentially expressed in endometrial cells and leukocytes during the menstrual cycle, in early pregnancy and in women using progestin-only contraception

Inhibins and activins are dimeric hormones which share common subunits and which have diverse endocrine and paracrine roles in regulating reproductive function. Endometrial expression of inhibin alpha, betaA and betaB subunit s was examined by immunohistochemistry and in-situ hybridization, across th e menstrual cycle and in early pregnancy. All three subunits were found to be expressed in endometrium, primarily by glandular epithelium in the early stages of the cycle. Following the onset of decidualization, expression of alpha, betaA and pe subunits was up-regulated in decidualized stromal cell s. A marked down-regulation of alpha subunit was detected in glandular epit helium, whilst expression of betaA and betaB subunits was maintained. This pattern was consistent in decidua from early pregnancy and additionally in endometrium from women using progestin-only contraceptives, either subderma l implants (Norplant(R)) or levonorgestrel-releasing intrauterine systems ( Lng-IUS). Immunostaining was also observed for both betaA and betaB subunit s in subpopulations of endometrial leukocytes, identified to be distinct su bsets of macrophages, neutrophils and mast cells. potential paracrine roles for activins may be envisaged in facilitating tissue remodelling during de cidualization, in tissue repair following menstruation, and additionally in modulating premenstrual inflammatory events.