Hemodialysis does not influence the peroxidative state already present in uremia

Hemodialysis (HD) patients are exposed to high oxidative stress, however, t he nature of this stress is still unclear. In this study, we employed a spe cific lipid peroxidative product, phosphatidylcholine hydroperoxide (PCOOH) , and evaluated the peroxidative effect of end stage renal disease by measu ring thiobarbituric acid reactive substances (TBARS) and PCOOH in both plas ma and erythrocyte membrane. We also surveyed plasma TBARS and PCOOH before and after HD sessions thereby assessing oxidative stress by a single HD pr ocedure. The plasma TBARS level of healthy controls was 2.9 +/- 0.4 nmol/ml . Those of HD patients before and after HD session were 5.1 +/- 1.4 and 3.1 +/- 0.5 nmol/ml, respectively, and the pre-HD plasma TBARS levels were sig nificantly higher than those of controls and after HD. The Plasma PCOOH con centration of patients before HD was 119.7 +/- 58.4 pmol/ml and was signifi cantly higher than that of controls which was 88.6 +/- 14.3 pmol/ml. After HD, the plasma PCOOH level decreased to 103.2 +/- 36.0 pmol/ml, which was s till significantly higher than that of controls. In erythrocytes, the PCOOH level of patients was 259.3 +/- 105.4 nmol/g RBC and was significantly hig her than that of controls with 88.6 +/- 32.0 nmol/g REC. Analyzed with resp ect to the cause of renal disease, the polycystic kidney disease patients s howed significantly lower plasma PCOOH levels than the others. These result s suggest that there is an increase of lipid peroxidation in both plasma an d erythrocytes of HD patients, though this oxidative stress was not brought about by HD. Copyright (C) 2000 S. Karger AG, Basel.