Cloning and expression of MP13 gene from rat hippocampus, a new factor related to guanosine triphosphate regulation

C-Fos and the Fos-related antigens (FRA) are induced by various stimuli. A novel 35-37 kDa FRA was induced much longer after the treatment using kaini c acid (KA) and may be very important for neuronal survival after brain dam age. To identify this long-term FRA, we have constructed a cDNA library der ived from hippocampus after KA treatment and screened it with an antibody h ighly conserved M-peptide region of FRAs. One gene, MP13, was cloned with a 1662 bp open reading frame and coded for a 554-amino acid protein. MP13 ha s a leucine zipper region, a glutamine repeat region, and has high similari ty to the activator of the small guanosine triphosphate (GTP)ase Rab5. Gel retardation analysis revealed that MP13 functions as a GTP regulation relat ed factor. (C) 2000 Elsevier Science Ireland Ltd. AII rights reserved.