d(GA center dot TC)(n) microsatellite DNA sequences enhance homologous DNArecombination in SV40 minichromosomes

The genomic distribution of the abundant eukaryotic d(GA.TC)(n) DNA microsa tellite suggests that it could contribute to DNA recombination. Here, it is shown that this type of microsatellite DNA sequence enhances DNA recombina tion in SV40 minichromosomes, the rate of homologous DNA recombination incr easing by as much as two orders of magnitude in the presence of a d(GA.TC)( 22) sequence. This effect depends on the region of the SV40 genome at which the d(GA.TC)(22) sequence is cloned. It is high when the sequence is locat ed proximal to the SV40 control region but no effect is observed when locat ed 3.5 kb away from the SV40 ori. These results indicate that the recombina tion potential of d(GA.TC)(n) sequences is likely linked to DNA replication and/or transcription. The potential contribution of the structural propert ies of d(GA.TC)(n) sequences to this effect is discussed.