Synovial inflammation and hyperplasia induced by gliostatin/platelet-derived endothelial cell growth factor in rabbit knees

Citation
Y. Waguri-nagaya et al., Synovial inflammation and hyperplasia induced by gliostatin/platelet-derived endothelial cell growth factor in rabbit knees, RHEUM INTL, 20(1), 2000, pp. 13-19
Citations number
27
Categorie Soggetti
Rheumatology,"da verificare
Journal title
RHEUMATOLOGY INTERNATIONAL
ISSN journal
01728172 → ACNP
Volume
20
Issue
1
Year of publication
2000
Pages
13 - 19
Database
ISI
SICI code
0172-8172(200012)20:1<13:SIAHIB>2.0.ZU;2-L
Abstract
Neovascularization, proliferation of synovial cells, and mononuclear cell i nflux and activation are characteristic events observed in synovial joints in the pathohistology of rheumatoid arthritis (RA). The objective of this s tudy was to examine synovial inflammation in rabbit knees induced by intra- articular administration of human gliostatin/platelet-derived endothelial c ell growth factor (GLS/PD-ECGF), which shares a high degree of chemical hom ology with thymidine phosphorylase (dThdPase) and is known to have angiogen ic activity. Purified recombinant human gliostatin (rHuGLS) and its mutant protein, which was prepared by site-directed mutagenesis and which lacks dT hdPase activity, were administered at various doses to rabbit knee joints. The effects of rHuGLS and the mutant were examined histologically. Intra-ar ticular injection of rHuGLS resulted in the development of diffuse synoviti s resembling RA. The mutant protein also brought about the same effect. The se findings suggest that human GLS carl cause RA-like synovitis in rabbit k nee joints via a mechanism other than its dThdPase activity.