Significance and features of MLC reaction in determination of histocompatibility of donor and recipient in allogenic bone marrow transplantation to patients with hematological malignancies

Aim. To characterize mixed lymphocyte culture (MLC) reaction used for deter mination of donor-recipient compatibility before bone marrow transplantatio n to patients with hematological malignancies and to assess the reaction si gnificance. Material and methods. The analysis was made of compatibility testing in MLC standard reaction performed in 134 patients with hematological malignancie s with HLA-A, -B identical donors-sibs and in 5 patients with haploid ident ical donors. Results. Out of 134 patients, 22(91%) appeared compatible to donor sibs in MLC reaction, 12(9%) patients were incompatible. Mean RR for MLC-compatible couples made up: in RvD direction 77 +/- 0.17%, DvR 2.61 +/- 0.32%. 93% of RR values ranged from +15 to -15%, the rest - from +25% to -25%. Bone marr ow transplantation was made in 83 patients. Graft retention was observed in 77(93%) patients. Acute and chronic graft versus host reaction developed i n 15 and 17 patients, respectively. Conclusion. An optimal protocol is proposed for examination of compatibilit y donor-recipient in MLC reaction in patients with hematological malignanci es. It is intended for allogenic bone marrow transplantation in hematologic al departments.