TB PCR in the early diagnosis of tuberculous meningitis: evaluation of theRoche semi-automated COBAS Amplicor MTB test with reference to the manual Amplicor MTB PCR test

Setting: Cecilia Makiwane Hospital, Mdantsane, Eastern Cape, Republic of So uth Africa. Objective: To assess the role of the semi-automated Roche COBAS AMPLICOR(TM ) Mycobacterium tuberculosis PCR test in the diagnosis of tuberculous menin gitis (TBM). Design: Eighty-three specimens of cerebrospinal fluid (CSF) were collected prospectively from 69 patients with suspected TBM. The COBAS AMPLICOR TB PC R test was compared with the manual AMPLICOR(TM) TB PCR test, clinical and cerebrospinal fluid (CSF) findings, direct ZN smear and radiometric TB cult ure. Results: CSF from 7/40 (17.5%) patients treated for TBM were positive by TB COBAS AMPLICOR(TM). The sensitivity of the test was not significantly diff erent (p = 0.375) from the manual TB AMPLICOR(TM) PCR test. The comparative sensitivities of the TB COBAS AMPLICOR(TM) PCR and the manual AMPLICOR PCR for detecting cases of definite and probable TBM from CSF collected within 9 days of commencing antituberculosis treatment were 40% and 60% respectiv ely. All 29 patients not treated for TBM were negative by COBAS AMPLICOR(TM ), giving a specificity of 100%. Conclusion: The COBAS AMPLICOR(TM) TB PCR test is a rapid and highly specif ic diagnostic test for TBM. However, there was a non-significant trend favo uring slightly greater sensitivity using the manual AMPLICOR(TM) TB PCR tes t. (C) 2000 Harcourt Publishers Ltd.