Short-term K+ deprivation provokes insulin resistance of cellular K+ uptake revealed with the K+ clamp

We aimed to test the feasibility of quantifying insulin action on cellular K+ uptake in vivo in the conscious rat by measuring the exogenous K+ infusi on rate needed to maintain constant plasma K+ concentration ([K+]) during i nsulin infusion. In this "K+ clamp" the K+ infusion rate required to clamp plasma [K+] is a measure of insulin action to increase net plasma K+ disapp earance. K+ infusion rate required to clamp plasma [K+] was insulin dose de pendent. Renal K+ excretion was not significantly affected by insulin at a physiological concentration (similar to 90 muU/ ml, P > 0.05), indicating t hat most of insulin-mediated plasma K+ disappearance was due to K+ uptake b y extrarenal tissues. In rats deprived of K+ for 2 days, plasma [K+] fell f rom 4.2 to 3.8 mM, insulin-mediated plasma glucose clearance was normal, bu t insulin-mediated plasma K+ disappearance decreased to 20% of control, eve n though there was no change in muscle Na-K-ATPase activity or expression, which is believed to be the main K+ uptake route. After 10 days K+ deprivat ion, plasma [K+] fell to 2.9 mM, insulin-mediated K+ disappearance decrease d to 6% of control (glucose clearance normal), and there were 50% decreases in Na-K-ATPase activity and alpha2-subunit levels. In conclusion, the pres ent study proves the feasibility of the K+ clamp technique and demonstrates that short-term K+ deprivation leads to a near complete insulin resistance of cellular K+ uptake that precedes changes in muscle sodium pump expressi on.