HYDROLYSIS AND ISOMERIZATION OF THE INTERNUCLEOSIDIC PHOSPHODIESTER BONDS OF POLYURIDYLIC ACID - KINETICS AND MECHANISM

Authors
KUUSELA S LONNBERG H
Citation
S. Kuusela et H. Lonnberg, HYDROLYSIS AND ISOMERIZATION OF THE INTERNUCLEOSIDIC PHOSPHODIESTER BONDS OF POLYURIDYLIC ACID - KINETICS AND MECHANISM, Perkin transactions. 2, (10), 1994, pp. 2109-2113
Citations number
28
Categorie Soggetti
Chemistry Physical","Chemistry Inorganic & Nuclear
Journal title
Perkin transactions. 2 → ACNP
ISSN journal
03009580
Issue
10
Year of publication
1994
Pages
2109 - 2113
Database
ISI
SICI code
0300-9580(1994):10<2109:HAIOTI>2.0.ZU;2-8
Abstract
The pH-rate profiles for the hydrolysis of the 3',5'-phosphodiester bo nds of poly(U) and their isomerization to the 2',5' bonds have been de termined by following the progress of the reactions by enzymatic diges tion with RNAase A and subsequent RP HPLC. The kinetics observed are c ompared with those of the hydrolysis and interconversion of uridylyl ( 3'.5')uridine and uridylyl(2',5') uridine.