Kinetics of enzymatic solid-to-solid peptide synthesis: Synthesis of Z-aspartame and control of acid-base conditions by using inorganic salts

Enzymatic peptide synthesis can be carried out efficiently in solid-to-soli d reaction mixtures with 10% (w/w) water added to a mixture of substrates. The final reaction mass contains greater than or equal to 80% (by weight) o f product. This article deals with acid-base effects in such reaction mixtu res and the consequences for the enzyme. In the Thermoase-catalyzed synthes is of Z-Asp-Phe-OMe, the reaction rate is strongly dependent on the amount of basic salts added to the system. The rate increases 20 times, as the KHC O3 or K2CO3 added is raised 2.25-fold from an amount equimolar to the Phe-O Me . HCL starting material. With further increases in KHCO3 addition, the i nitial rate remains at the maximum, but with K2CO3 it drops sharply. Additi on of NaHCO3 is less effective, but rates are faster if more water is used. With >1.5 equivalents of basic salt, the final yield of the reaction decre ases. Similar effects are observed when thermolysin catalyzes the same reac tion, or Z-Gln-Leu-NH2, synthesis. These effects can be rationalized using a model estimating the pH of these systems, taking into account the possibl e formation of up to ten different solid phases. (C) 2001 John Wiley & Sons , Inc.